Variation in the Efficacy of Several Predator Gut Content Immunoassays
نویسنده
چکیده
Five different immunoassay formats were examined for their ability to detect a minute quantity of prey remains in predator guts. The convergent lady beetle, Hippodamia convergens Guérin-Meneville, that had consumed either one or five pink bollworm, Pectinophora gossypiella (Saunders), eggs was evaluated by the following immunoassays: three variations of enzyme-linked immunosorbent assay (ELISA), a dot blot, and a Western blot. Sandwich ELISA, dot blot, and Western blot were the most sensitive immunoassays based on the proportion of individual predators scoring positive for prey remains. The direct ELISA and indirect ELISA were ineffective at detecting prey in the predators. The advantages and disadvantages of each immunoassay format are discussed.
منابع مشابه
Measuring the Sensitivity of an Indirect Predator Gut Content ELISA: Detectability of Prey Remains in Relation to Predator Species, Temperature, Time, and Meal Size
The gut contents of three species of insect predators that were fed either a variable or a fixed number of pink bollworm eggs but held at variable time and temperature regimes were assayed by an indirect enzyme-linked immunosorbent assay (ELISA). The sensitivity and efficacy of the monoclonal antibody-based ELISA was dependent on the predator species examined. Small predatorsweremore immunoresp...
متن کاملPerformance characteristics of three automated immunoassays for thyroid hormones
Background: Since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. We performed an analytical evaluation of the new electrochemiluminescent immunoassay (ECLIA) for serum TSH, FT4 and T3 in the Elecsys 2010 immunoassay system and compared the resu...
متن کاملMolecular gut-content analysis of a predator assemblage reveals the effect of habitat manipulation on biological control in the field
Habitat manipulation in agroecosystems can influence predator–prey interactions. In this study, we collected foliar predators from field potato plots with different mulch treatments and assayed them for DNA of the target prey, Leptinotarsa decemlineata (Say), using species-specific primers. Concurrently, L. decemlineata larval abundance and plant damage were recorded from the same plots. Predat...
متن کاملToward the Quantification of Predation with Predator Gut Immunoassays: A New Approach Integrating Functional Response Behavior
t f m Immunological methods have been widely used to identify key predator species and qualitatively evaluate predation of target prey. However, despite the quantitative nature of many immunoassays, the translation to number of prey attacked has been problematic because of the many factors that confound interpretation of the strength of the immunoassay response. We developed a new predation mod...
متن کاملDynamics of Food Chain Model: Role of Alternative Resource for Top Predator
In this paper, effect of alternative resource for top predator in food chain model with holling type III functional response is seen . Proposed model is demonstrated in respect of analytical as well numerical results. Bifurcation study with the variation of alternative resource and half saturation constants are done numerically. Simulation results shows that suitable alternati...
متن کامل